Information-enriched characterization of cell populations. Morphological information and facts from pictures can add into the richness of flow cytometry information. For instance, the determine shows an if not traditional apoptosis assay employing Annexin V and PI, adding cell imaging to characterize cells in Every single inhabitants to reveal morphologically unique characteristics. These insights could not have been received from multiplex staining by yourself.
As samples are obtained over the Attune CytPix Flow Cytometer, the substantial-pace brightfield camera captures and outlets illustrations or photos of detected occasions in a level as much as 6,000 photographs per second, depending upon the flow fee and picture dimensions. For larger flexibility, Attune Cytometric Program helps you to regulate the graphic seize frequency as required and select a specific gate for image seize, if demanded.
Blend acoustic concentrating technologies with higher-parameter Evaluation for adaptable spectral unmixing and traditional workflows.
The award-profitable Invitrogen Attune CytPix Flow Cytometer combines acoustic focusing using a significant-speed brightfield camera for simultaneous significant throughput flow cytometry and higher resolution brightfield imaging.
*Pricing is indicative only and is matter to alter. You should contact your Agilent consultant with any issues. The knowledge established forth herein is not really an offer or a commitment, each of that happen to be matter to credit history approval, conditions and terms, and availability with Agilent. Agilent Monetary Options is available in chosen nations around the world.
Also, it really is illuminating to view what number of doublets slip by means of regular singlet gates, and terrific to confirm the status of unpredicted double-positive occasions in my Investigation."
Optimizing gating method. Even strong guide singlet gating is mistake-inclined and remains a subjective selection place in Virtually all flow cytometry assays. Imaging can be employed to substantiate and change gates to incorporate only solitary cells of desire.
Our experts will style and design panels for yourself as persistently you will need—totally free, no product invest in needed.
Early signs of this contamination experienced originally been dismissed as debris. Backgating to find out new populations. Backgating imaged cells over the Attune CytPix Flow Cytometer also enables you to use morphological options to find out exciting subpopulations that might not be apparent from flow cytometry details alone.
Perhaps most of all, these functions consist of cells of Plainly distinct phenotypes which could lead to incorrect conclusions relating to double optimistic gatherings (particularly in exceptional populations).
Sound waves enable specific mobile alignment, leading to exceptional facts top quality and ~10X more quickly sample flow rates.
Application Be aware: Automated graphic analysis minimizes person-to-user variability in flow cytometry gating tactics
Created-in dimensionality reduction equipment elevated resolution into populations. Fluorescently gated populations back-gated onto UMAP plot to substantiate anticipated inhabitants resolution in lysed whole human blood. Person can look at events from Every cluster for Visible affirmation of cell phenotypes.
Adaptable payment approach from Agilent Economic Options check here help you receive the vital instrumentation you'll need for speedy venture, or to grow your lab.
Gating on these prolonged parameters helps you to quickly and accurately recognize populations of desire and ensure gating system with little or no manual critique. The Attune application presents access to state-of-the-artwork equipment Studying Evaluation tools within just an expertly made user interface, which makes it exceptionally simple to operate.
The Attune Xenith cytometer can be a higher-parameter spectral analyzer, giving both spectral unmixing and standard flow capabilities for extensive and versatile cell Examination.
Agilent Benefit Guarantee ensures you no less than ten years of instrument use through the day you purchase. In any other case, we will credit history you Using the residual price of the process towards an upgraded model.
What appeared to be a homogeneous population is actually much more various—an insight that should be considered when interpreting results.